PIK3R2 antibody (AA 520-720)

Catalog No. ABIN7126793

This Mouse Monoclonal antibody specifically detects PIK3R2 in IHC. It exhibits reactivity toward Human.

Quick Overview for PIK3R2 antibody (AA 520-720) (ABIN7126793)

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIK3R2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: PIK3R2-292

Product Details anti-PIK3R2 Antibody

Binding Specificity: AA 520-720

Purpose: PIK3R2 / Phosphoinositide-3-kinase regulatory subunit 2 Antibody

Specificity: Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85°™ and p85 æ), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110°™ and p110 æ interact with p85°™, and p110°™ has also been shown to interact with p85 æ in vitro. p110 79oΩL expression is restricted to white blood cells. It has been shown to bind p85°™ and p85 æ, but it apparently does not phosphorylate these subunits. p110 79oΩL seems to have the capacity to autophosphorylate. p110Á does not interact with the p85 subunits. It has been shown to be activated by °™ and æÁ heterotrimeric G proteins.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human PIK3R2 protein (around aa 520-720) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human spleen or bladder.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PIK3R2

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Alternative Name: PIK3R2

Background:

p85 beta, P85B, Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta), Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta), PI3 kinase p85 beta subunit, PI3K regulatory subunit beta, PIK3R2, PtdIns-3-kinase regulatory subunit p85-beta

Human spleen or bladder.

Molecular Weight: 85kDa

Gene ID: 5296, 371344

UniProt: O00459

Pathways: VEGF Signaling, BCR Signaling