MBNL3 antibody

Catalog No. ABIN7126833

Product Details anti-MBNL3 Antibody

Target: MBNL3 (Muscleblind-Like Splicing Regulator 3 (MBNL3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBNL3 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression. Several protein complexes are involved in proper mRNA splicing and transport. The muscleblind proteins, MBNL1, MBNL2 and MBNL3, promote inclusion or exclusion of specific exons on different pre-mRNAs by antagonizing the activity of CUG-BP and ETR-3-like factors bound to distinct intronic sites. MBNL1 and 2, which associate with expanded CUG repeats in vitro, localize to the nuclear foci in both DM1 and DM2 (myotonic dystrophy types 1 and 2), suggesting that the nuclear accumulation of mutant RNA is pathogenic in DM1, therefore implicating MBNL1 and 2 in the pathogenesis of both disorders. MBNL3, a 354 amino acid protein, inhibits expression of muscle differentiation, opposite to the function of MBNL1, which functions as a promoter of muscle differentiation. MBNL3 shows strong expression in placenta.

Cross-Reactivity (Details): Human

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MBNL3 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF7 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MBNL3

Target: MBNL3 (Muscleblind-Like Splicing Regulator 3 (MBNL3))

Alternative Name: MBNL3 (MBNL3 Products)

Synonyms: A530038J18Rik antibody, AI661274 antibody, CHCR antibody, E430034C16Rik antibody, MBLX antibody, MBLX39 antibody, MBXL antibody, mbxl antibody, RGD1565834 antibody, muscleblind like splicing regulator 3 antibody, muscleblind like splicing factor 3 antibody, Muscleblind-like protein 3-like antibody, muscleblind-like splicing regulator 3 antibody, MBNL3 antibody, Mbnl3 antibody, mbnl3 antibody

Background: CHCR, Cys3His CCG1 required protein, MBLX, MBLX39, Muscleblind like protein 3, Muscleblind like splicing regulator 3, Muscleblind-like X-linked protein, Protein HCHCR, RP23-98P13.1,MBNL3
Cellular localisation: Nucleus. Cytoplasm. Greater concentration in the nucleus. In both DM1 and DM2 patients, colocalizes with nuclear foci of retained expanded-repeat transcripts.

Molecular Weight: 39kDa

Gene ID: 55796

UniProt: Q9NUK0

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development