MBNL3 antibody

Catalog No. ABIN7861760

This Mouse Monoclonal antibody specifically detects MBNL3 in FACS. It exhibits reactivity toward Human.

Quick Overview for MBNL3 antibody (ABIN7861760)

Target: MBNL3 (Muscleblind-Like Splicing Regulator 3 (MBNL3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBNL3 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: PCRP-MBNL3-1D11

Product Details anti-MBNL3 Antibody

Purpose: MBNL3 Antibody

Specificity: Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression. Several protein complexes are involved in proper mRNA splicing and transport. The muscleblind proteins, MBNL1, MBNL2 and MBNL3, promote inclusion or exclusion of specific exons on different pre-mRNAs by antagonizing the activity of CUG-BP and ETR-3-like factors bound to distinct intronic sites. MBNL1 and 2, which associate with expanded CUG repeats in vitro, localize to the nuclear foci in both DM1 and DM2 (myotonic dystrophy types 1 and 2), suggesting that the nuclear accumulation of mutant RNA is pathogenic in DM1, therefore implicating MBNL1 and 2 in the pathogenesis of both disorders. MBNL3, a 354 amino acid protein, inhibits expression of muscle differentiation, opposite to the function of MBNL1, which functions as a promoter of muscle differentiation. MBNL3 shows strong expression in placenta.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human MBNL3 protein

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MBNL3

Target: MBNL3 (Muscleblind-Like Splicing Regulator 3 (MBNL3))

Alternative Name: MBNL3

Background:

CHCR, Cys3His CCG1 required protein, MBLX, MBLX39, Muscleblind like protein 3, Muscleblind like splicing regulator 3, Muscleblind-like X-linked protein, Protein HCHCR, RP23-98P13.1

HeLa or MCF7 cells.

Molecular Weight: 39kDa

Gene ID: 55796

UniProt: Q9NUK0

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development