RBP4 antibody (AA 29-148)

Catalog No. ABIN7126875

The Mouse Monoclonal anti-RBP4 antibody has been validated for IHC (f). It is suitable to detect RBP4 in samples from Human.

Quick Overview for RBP4 antibody (AA 29-148) (ABIN7126875)

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RBP4 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-RBP4 Antibody

Binding Specificity: AA 29-148

Specificity: Retinol (Vitamin A) is transported in the blood bound to its carrier protein, retinol-binding protein (RBP), also designated plasma retinol-binding protein (PRBP) or RBP4. A member of the lipocalin family, RBP conveys retinol from stores in the liver to peripheral tissues. In plasma, RBP binds transthyretin (TTR, formerly called prealbumin) to prevent glomerular filtration of low molecular weight RBP in the kidneys. The stability of this complex holds diagnostic importance because the molar ratio of RBP:TTR provides an indirect way to indicate marginal Vitamin A deficiency. Vitamin A deficiency blocks the secretion of RBP, resulting in defective delivery and supply to epidermal cells. Originally identified solely as a transporter protein, recent studies correlating increased levels of RBP expression in adipose tissue with Insulin resistance have generated research into the possible roles the protein may play in the pathogenesis of type 2 diabetes and obesity.

Cross-Reactivity (Details): Human

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa29-148) of human RBP4 protein (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeP-G2 cells. Human kidney, pancreas or liver tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for RBP4

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Alternative Name: RBP4

Background: Plasma retinol binding protein 4 (PRBP), RDCCAS, Retinol binding protein 4 interstitial,RBP4 / Retinol Binding Protein 4
Cellular localisation: Secreted

Molecular Weight: 25kDa

Gene ID: 5950, 50223

UniProt: P02753

Pathways: Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response