SOD1 antibody (AA 14-148)

Catalog No. ABIN7126954

The Mouse Monoclonal anti-SOD1 antibody has been validated for WB, IF, FACS and IHC (f). It is suitable to detect SOD1 in samples from Human.

Quick Overview for SOD1 antibody (AA 14-148) (ABIN7126954)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-SOD1 Antibody

Binding Specificity: AA 14-148

Specificity: Cu-Zn superoxide dismutase-1 (SOD-1) is a well-characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Enzymatically, SOD-1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. SOD-1 is ubiquitously expressed in somatic cells and functions as a homodimer. Defects in the gene encoding SOD-1 have been implicated in the progression of neurological diseases, including amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by the loss of spinal motor neurons, Down syndrome and Alzheimer s disease. In familial ALS, several mutations in SOD-1 predominate, resulting in the loss of zinc binding, the loss of scavenging activity of SOD-1, and correlate with an increase in neurotoxicity and motor neuron death.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa14-148) of human SOD1 (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: JEG-3 or Jurkat cells. Human breast or ovarian carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Alternative Name: SOD1

Background: Amyotrophic lateral sclerosis 1 (ALS1), Cu/Zn SOD, Cu/Zn Superoxide Dismutase, Epididymis Secretory Protein Li 44, Indophenoloxidase A (IPOA), Superoxide Dismutase [Cu-Zn], Superoxide Dismutase 1 (SOD1),Superoxide Dismutase 1 (SOD1) (Antioxidant Enzyme)
Cellular localisation: Cytoplasmic

Molecular Weight: 16kDa

Gene ID: 6647, 443914

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis