Recombinant STAR antibody (AA 39-108)

Catalog No. ABIN7126984

The Rabbit Monoclonal anti-STAR antibody has been validated for IHC (f). It is suitable to detect STAR in samples from Human.

Quick Overview for Recombinant STAR antibody (AA 39-108) (ABIN7126984)

Target: STAR (Steroidogenic Acute Regulatory Protein (STAR))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This STAR antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-STAR Antibody

Binding Specificity: AA 39-108

Specificity: Steroidogenic Acute Regulatory Protein (STAR) controls the rate-limiting step of steroidegenesis by translocating cholesterol from the outer mitochondrial membrane to the inner membrane where it is later cleaved to pregnenolone.It is primarily present in steroid-producing cells, including Leydig cells in the testis, theca cells and luteal cells in the ovary and adrenal cells in the adrenal cortex. Due to low levels of pregnenolone, seminomas and Leydig cell tumors display no specific STAR staining. Therefore, STAR antibody may assist in differentiating sex cord stromal tumors (SCST), seminomas and embryonal carcinomas.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 39-108) of human STAR protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: K-562 cells. Human adrenal or testicular carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for STAR

Target: STAR (Steroidogenic Acute Regulatory Protein (STAR))

Alternative Name: STAR

Background: Cholesterol trafficker, Luteinizing hormone induced protein, Mitochondrial steroid acute regulatory protein, StAR, StAR related lipid transfer (START) domain containing 1, StARD1, START domain-containing protein 1, Steroid acute regulatory protein, Steroidogenic acute regulator (STAR), Steroidogenic acute regulatory protein mitochondrial,StAR (Steroidogenic Acute Regulator) (Leydig Cell Marker)
Cellular localisation: Cytoplasm (Mitochondria).

Molecular Weight: 30kDa

Gene ID: 6770, 521535

UniProt: P49675

Pathways: Metabolism of Steroid Hormones and Vitamin D, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis