MED22 antibody

Catalog No. ABIN7126989

Product Details anti-MED22 Antibody

Target: MED22 (Mediator Complex Subunit 22 (MED22))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MED22 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med22 (mediator complex subunit 22), also known as SURF5 (surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex. There are two isoforms of Med22 that are produced as a result of alternative splicing events.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MED22 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunofluorescence (0.5-2 μg/mL), Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (0.5-2 μg/million cells), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or U87 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MED22

Target: MED22 (Mediator Complex Subunit 22 (MED22))

Alternative Name: MED22 (MED22 Products)

Synonyms: MED24 antibody, SURF5 antibody, surf-5 antibody, AW212655 antibody, AW558812 antibody, Surf-5 antibody, Surf5 antibody, 144358_at antibody, CG18830 antibody, CG3034 antibody, Dmel\\CG3034 antibody, EG:BACR7A4.6 antibody, Med22 antibody, Med24 antibody, Srb6/Surf5 antibody, anon-EST:Liang-1.82 antibody, clone 1.82 antibody, dMED24 antibody, si:ch73-278c20.1 antibody, si:dkey-226m8.2 antibody, surf5 antibody, wu:fj45h07 antibody, zgc:86721 antibody, Surf5b antibody, MGC80238 antibody, RGD1564893 antibody, mediator complex subunit 22 antibody, Mediator complex subunit 22 antibody, mediator complex subunit 22 L homeolog antibody, MED22 antibody, Med22 antibody, med22 antibody, med22.L antibody

Background: Med24, SURF5, mediator complex subunit 22, Mediator of RNA polymerase II transcription subunit 22,MED22 (Transcription Factor)
Cellular localisation: Nucleus.

Molecular Weight: 22kDa

Gene ID: 6837, 78354

UniProt: Q15528

Pathways: Regulation of Lipid Metabolism by PPARalpha