MED22 antibody

Catalog No. ABIN7863437

The Mouse Monoclonal anti-MED22 antibody is suitable to detect MED22 in samples from Human. It has been validated for ELISA, WB, IF and FACS.

Quick Overview for MED22 antibody (ABIN7863437)

Target: MED22 (Mediator Complex Subunit 22 (MED22))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MED22 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-MED22-1E4

Product Details anti-MED22 Antibody

Purpose: MED22 (Transcription Factor) Antibody

Specificity: Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med22 (mediator complex subunit 22), also known as SURF5 (surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex. There are two isoforms of Med22 that are produced as a result of alternative splicing events.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human MED22 protein

Isotype: IgG2c

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MED22

Target: MED22 (Mediator Complex Subunit 22 (MED22))

Alternative Name: MED22

Background:

Med24, SURF5, mediator complex subunit 22, Mediator of RNA polymerase II transcription subunit 22

HeLa or U87 cells.

Molecular Weight: 22kDa

Gene ID: 6837, 78354

UniProt: Q15528

Pathways: Regulation of Lipid Metabolism by PPARalpha