Recombinant MSH2 antibody (AA 224-313)

Catalog No. ABIN7126990

The Mouse Monoclonal anti-MSH2 antibody has been validated for IHC (f). It is suitable to detect MSH2 in samples from Human.

Quick Overview for Recombinant MSH2 antibody (AA 224-313) (ABIN7126990)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH2 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-MSH2 Antibody

Binding Specificity: AA 224-313

Specificity: This monoclonal antibody recognizes a protein of 38 kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 224-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, HeLa or Y79 cells. Human pancreas, cerebellum or pheochromocytoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: MSH2

Background: Major synaptic vesicle protein p38, MRX96, MRXSYP, Syn p38, Syp, SYPH,Synaptophysin (Neuroendocrine Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 38kDa

Gene ID: 6855, 632804

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response