TIA1 antibody (AA 279-380)

Catalog No. ABIN7127001

This Mouse Monoclonal antibody specifically detects TIA1 in WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for TIA1 antibody (AA 279-380) (ABIN7127001)

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TIA1 Antibody

Binding Specificity: AA 279-380

Specificity: TIA-1 (T-cell intracytoplasmic antigen) is a cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. TIA-1 is a member of an RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15 kDa protein thought to be derived from the carboxyl terminus of the 40 kDa product by proteolytic processing. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human TIA1 fragment of 102 amino acid residues (aa279-380)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL for 60 minutes at RT),Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, PC3 or HeLa cells. Human tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TIA1

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Alternative Name: TIA1

Background: Cytotoxic granule associated RNA binding protein 1, mTIA-1, Nucleolysin TIA1 isoform p40, p40-TIA-1, RNA binding protein TIA1, T-cell-restricted intracellular antigen-1 (TIA1), TIA-1, TIA1, TIA1 cytotoxic granule associated RNA binding protein, TIAL1, TIAR, WDM,TIA1 (T-Cell-Restricted Intracellular Antigen-1)
Cellular localisation: Cytoplasmic

Molecular Weight: 40kDa

Gene ID: 7072, 413123

UniProt: P31483