TNFRSF4 antibody (AA 59-205)

Catalog No. ABIN7127032

Product Details anti-TNFRSF4 Antibody

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Binding Specificity: AA 59-205

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF4 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This MAb recognizes a protein of 43 kDa, identified as OX40, which is also known as CD134.OX40 is a type I integral membrane glycoprotein and member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family. It is expressed on activated T lymphocytes, hematopoietic precursor cells and fibroblasts. It functions as a T cell co-stimulatory receptor when bound by OX40 Ligand / TNFSF4 that is expressed by antigen presenting cells. OX40 thereby plays roles in T-cell activation as well as the regulation of differentiation, proliferation or apoptosis of normal and malignant lymphoid cells.OX40 is upregulated at the sites of inflammation, especially in case of multiple sclerosis and psoriatic lesions.

Cross-Reactivity (Details): Human and Chimpanzee.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human OX40 (CD134) protein (around aa 59-205) (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HuT78 cells or human peripheral blood leukocytes (hPBL). Human tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TNFRSF4

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Alternative Name: TNFRSF4 (TNFRSF4 Products)

Background: ACT35, CD134, Lymphoid activation antigen ACT35, OX40, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4 (Tumor necrosis factor receptor superfamily member 4),OX40 / CD134 / TNFRSF4 (Immuno-Oncology Target)
Cellular localisation: Cell Surface

Molecular Weight: 43kDa

Gene ID: 7293, 129780

UniProt: P43489

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints