Recombinant Vimentin antibody (AA 366-466)

Catalog No. ABIN7127053

The Rabbit Monoclonal anti-Vimentin antibody has been validated for WB and IHC (f). It is suitable to detect Vimentin in samples from Human.

Quick Overview for Recombinant Vimentin antibody (AA 366-466) (ABIN7127053)

Target: Vimentin (VIM)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Vimentin Antibody

Binding Specificity: AA 366-466

Specificity: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa366-466) of human Vimentin protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Western Blot (@-4 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U-87, Raji, Jurkat or HeLa cells. Human sarcomas ormelanomas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: VIM

Background: VIM,Vimentin (Mesenchymal Cell Marker)
Cellular localisation: Cytoplasm

Molecular Weight: 57-60kDa

Gene ID: 7431, 455493

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis