BAP1 antibody (AA 191-326)

Catalog No. ABIN7127089

The Mouse Monoclonal anti-BAP1 antibody is suitable to detect BAP1 in samples from Human. It has been validated for IHC (f).

Quick Overview for BAP1 antibody (AA 191-326) (ABIN7127089)

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAP1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-BAP1 Antibody

Binding Specificity: AA 191-326

Specificity: The BAP1 gene belongs to the ubiquitin C-terminal hydrolase subfamily of de-ubiquitination enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germ line mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, Uveal melanoma and cutaneous melanoma.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human BAP1 protein fragment (around aa 191-326) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cell or PC-3 cell lysates. HepG2 cells. Human testis, placenta, ovaries and breast.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for BAP1

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Alternative Name: BAP1

Background: BAP1, BRCA1 associated protein 1, Cerebral protein 13, Cerebral protein 6, HUCEP 13, Hucep 6, HUCEP13, Hucep6, TPDS, Ubiquitin carboxy terminal hydrolase, Ubiquitin carboxyl terminal hydrolase BAP 1, UCHL2,BAP1 (BRCA1 Associated Protein 1)
Cellular localisation: Mainly nucleus, also cytoplasm. Binds to chromatin.

Molecular Weight: 91kDa

Gene ID: 8314, 106674

UniProt: Q92560