CD80 antibody (AA 35-142)

Catalog No. ABIN7127180

This anti-CD80 antibody is a Mouse Monoclonal antibody detecting CD80 in ELISA, Coat and IHC (f). Suitable for Human.

Quick Overview for CD80 antibody (AA 35-142) (ABIN7127180)

Target: CD80

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD80 antibody is un-conjugated

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CD80 Antibody

Binding Specificity: AA 35-142

Specificity: T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells bind the homologous T cell receptors CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and CD28 and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31 % overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counter receptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human CD80 protein (around aa 35-142) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Rajior Ramos cells. Human tonsil, spleen or thymus.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background: Activation B7-1 antigen, B lymphocyte activation antigen B7, B7, B7-1, BB1, CD28 antigen ligand 1, CD28LG, CD28LG1, CD80, Costimulatory factor CD80, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80,CD80 (B7-1)
Cellular localisation: Cell Surface

Molecular Weight: 60kDa

Gene ID: 941, 838

UniProt: P33681

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints