Recombinant Cytokeratin 2 antibody (AA 300-400)

Catalog No. ABIN7127252

The Mouse Monoclonal anti-Cytokeratin 2 antibody (Clone rKRTH-6617) (ABIN7127252) specifically detects Cytokeratin 2 in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Cytokeratin 2 antibody (AA 300-400) (ABIN7127252)

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RKRTH-6617

Product Details anti-Cytokeratin 2 Antibody

Binding Specificity: AA 300-400

Purpose: Recombinant Cytokeratin, Type II (Epithelial Marker) Antibody

Specificity: The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Synthetic peptide corresponding to Type II Cytokeratins residues within aa300-400 of Type II Cytokeratins

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Skin or squamous cell carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytokeratin 2

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Alternative Name: KRT76

Background:

KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9

Skin or squamous cell carcinoma.

Molecular Weight: 66/65/64/57/62/60/51/54kDa

Gene ID: 51350, 654392

UniProt: Q01546