Recombinant H2AFX antibody (pSer139)

Catalog No. ABIN7127309

This Rabbit Monoclonal antibody specifically detects H2AFX in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant H2AFX antibody (pSer139) (ABIN7127309)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 1F10

Product Details anti-H2AFX Antibody

Binding Specificity: pSer139

Cross-Reactivity: Human

Purification: Affinity-chromatography

Immunogen: A synthesized peptide

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:3000-1:10000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AFX

Background:

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Aliases: Histone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus