Recombinant PAK2 antibody

Catalog No. ABIN7127664

The Rabbit Monoclonal anti-PAK2 antibody has been validated for WB, ELISA, IP and FACS. It is suitable to detect PAK2 in samples from Human.

Quick Overview for Recombinant PAK2 antibody (ABIN7127664)

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PAK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 6D12

Product Details anti-PAK2 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PAK2

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:5000, FC:1:20-1:200, IP:1:200-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PAK2

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Alternative Name: PAK2

Background:

Background: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation.

Aliases: Serine/threonine-protein kinase PAK 2 (EC 2.7.11.1) (Gamma-PAK) (PAK65) (S6/H4 kinase) (p21-activated kinase 2) (PAK-2) (p58) [Cleaved into: PAK-2p27 (p27), PAK-2p34 (p34) (C-t-PAK2)], PAK2

UniProt: Q13177

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha