Recombinant PAK4 antibody (pSer474, pSer560, pSer602)

Catalog No. ABIN7127723

This Rabbit Monoclonal antibody specifically detects PAK4 in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for Recombinant PAK4 antibody (pSer474, pSer560, pSer602) (ABIN7127723)

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PAK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 2D1

Product Details anti-PAK4 Antibody

Binding Specificity: pSer474, pSer560, pSer602

Purpose: Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-PAK4/PAK5/PAK6 (S474/S560/S602)

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5000, IF:1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PAK4

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Alternative Name: PAK4

Background:

Background: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

Aliases: Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4, PAK4, KIAA1142

UniProt: O96013, Q9P286, Q9NQU5

Pathways: RTK Signaling