TNF alpha antibody (C-Term)

Catalog No. ABIN7127923

This anti-TNF alpha antibody is a Rabbit Polyclonal antibody detecting TNF alpha in WB, ELISA and IHC. Suitable for Human.

Quick Overview for TNF alpha antibody (C-Term) (ABIN7127923)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TNF alpha Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity purification

Immunogen: C term -peptide of Human SFPQ

Isotype: IgG

Application Details

Application Notes: WB:1:1000-1:2000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Store at -20 °C or -80 °C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF

Background:

Background: TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein. Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer. Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages. Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death. TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis. The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn's disease.

Aliases: APC1 antibody, APC1 protein antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, Tnf antibody, TNF superfamily member 2 antibody, TNF superfamily, member 2 antibody, TNF, macrophage derived antibody, TNF, monocyte derived antibody, TNF-a antibody, TNF-alpha antibody, TNFA antibody, TNFA_HUMAN antibody, TNFSF2 antibody, Tumor necrosis factor (TNF superfamily member 2) antibody, Tumor necrosis factor alpha antibody, Tumor necrosis factor antibody, Tumor necrosis factor ligand superfamily member 2 antibody, Tumor Necrosis Factor, Membrane Form antibody, Tumor necrosis factor, soluble form antibody

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome