His Tag antibody

Catalog No. ABIN7131650

This Mouse Monoclonal antibody specifically detects His Tag in WB and ELISA. It exhibits reactivity toward .

Quick Overview for His Tag antibody (ABIN7131650)

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 3G5H8

Product Details anti-His Tag Antibody

Purification: Protein A purified

Immunogen: HHHHHH(6x His) synthetic peptide conjugate to KLH

Isotype: IgG2a

Application Details

Application Notes: Recommended dilution: WB:1:500-1:2000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for His Tag

Target: His Tag

Alternative Name: 6*His

Target Type: Tag

Background:

Background: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.

Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag