CDK2 antibody (pThr14)

Catalog No. ABIN7138398

The Rabbit Polyclonal anti-CDK2 antibody is suitable to detect CDK2 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for CDK2 antibody (pThr14) (ABIN7138398)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CDK2 Antibody

Binding Specificity: pThr14

Purpose: Phospho-CDK2 (Thr14) Antibody

Cross-Reactivity: Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phospho rylation site of threonine14(E-G-T(p)-Y-G) derived from Human CDK1/CDC2 .

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2

Background:

Background:

Serine/threonine-protein kinase involved in the control of the cell cycle, essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression, controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus.

Sarkar S, Dey BK, Dutta A (2010) Mol Biol Cell 21, 2138-49
Shreeram S, Hee WK, Bulavin DV (2008) Mol Cell Biol 28, 7442-50
Mayya V, et al. (2006) Mol Cell Proteomics 5, 1146-57

Aliases: Cdc2 related protein kinase antibody, cdc2-related protein kinase antibody, CDC28 antibody, CDC2A antibody, Cdk 2 antibody, CDK1 antibody, CDK2 antibody, CDK2_HUMAN antibody, CDKN2 antibody, Cell devision kinase 2 antibody, Cell division protein kinase 2 antibody, Cyclin dependent kinase 2 antibody, cyclin dependent kinase 2-alpha antibody, Cyclin-dependent kinase 2 antibody, kinase Cdc2 antibody, MPF antibody, p33 protein kinase antibody, p33(CDK2) antibody

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA