BRCA1 antibody (pSer1457)

Catalog No. ABIN7138430

Product Details anti-BRCA1 Antibody

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Binding Specificity: pSer1457

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Human

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 1457 (L-T-S(p)-Q-K) derived from Human BRCA1.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1 (BRCA1 Products)

Background:

Background:

The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

Miki Y., Science 266:66-71(1994).
Smith T.M., Genome Res. 6:1029-1049(1996).
Wilson C.A., Oncogene 14:1-16(1997).

Aliases: BRCA 1 antibody, BRCA1 antibody, BRCA1 DNA repair associated antibody, BRCA1/BRCA2 containing complex subunit 1 antibody, BRCA1/BRCA2-containing complex, subunit 1 antibody, BRCA1_HUMAN antibody, BRCAI antibody, BRCC 1 antibody, BRCC1 antibody, Breast and ovarian cancer susceptibility protein 1 antibody, Breast Cancer 1 antibody, Breast Cancer 1 Early Onset antibody, Breast cancer type 1 susceptibility protein antibody, BROVCA1 antibody, FANCS antibody, IRIS antibody, PNCA4 antibody, PPP1R53 antibody, Protein phosphatase 1 regulatory subunit 53 antibody, PSCP antibody, RING finger protein 53 antibody, RNF53 antibody

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus