MSK1 antibody (pSer212)

Catalog No. ABIN7138480

The Rabbit Polyclonal anti-MSK1 antibody (ABIN7138480) specifically detects MSK1 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for MSK1 antibody (pSer212) (ABIN7138480)

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MSK1 Antibody

Binding Specificity: pSer212

Purpose: Phospho-RPS6KA5 (Ser212) Antibody

Cross-Reactivity: Mouse

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 212 (A-Y-S(p)-F-C) derived from Human MSK1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MSK1

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Alternative Name: RPS6KA5

Background:

Background:

Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).

McCoy CE, et al. (2007) Biochem J 402, 491-501
McCoy CE, et al. (2005)Biochem J 387, 507-17

Aliases: 90 kDa ribosomal protein S6 kinase 5 antibody, EC 2.7.11.1 antibody, KS6A5_HUMAN antibody, MGC1911 antibody, Mitogen and stress activated protein kinase 1 antibody, MSPK1 antibody, Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody, Nuclear mitogen- and stress-activated protein kinase 1 antibody, Ribosomal protein S6 kinase 90kD polypeptide 5 antibody, Ribosomal protein S6 kinase 90 kDa antibody, Ribosomal protein S6 kinase 90 kDa polypeptide 5 antibody, Ribosomal Protein S6 Kinase Alpha 5 antibody, Ribosomal protein S6 kinase alpha-5 antibody, RLPK antibody, RPS6KA5 antibody, RSK Like Protein Kinase antibody, RSK-like protein kinase antibody, RSKL antibody, S6K alpha 5 antibody, S6K-alpha-5 antibody

UniProt: O75582

Pathways: MAPK Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades