BLM antibody (pThr99)

Catalog No. ABIN7138888

The Rabbit Polyclonal anti-BLM antibody (ABIN7138888) specifically detects BLM in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for BLM antibody (pThr99) (ABIN7138888)

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-BLM Antibody

Binding Specificity: pThr99

Purpose: Phospho-BLM (Thr99) Antibody

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for BLM

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Alternative Name: BLM

Background:

Background:

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).

Ellis N.A., Cell 83:655-666(1995).
Karow J.K., J. Biol. Chem. 272:30611-30614(1997).
Pichierri P., EMBO J. 23:3154-3163(2004).

Aliases: Blm antibody, BLM_HUMAN antibody, Bloom syndrome antibody, Bloom syndrome protein antibody, Bloom syndrome RecQ helicase like antibody, BS antibody, DNA helicase antibody, DNA helicase RecQ like type 2 antibody, MGC126616 antibody, MGC131618 antibody, MGC131620 antibody, RECQ 2 antibody, RECQ like antibody, RecQ like type 2 antibody, RecQ protein like 3 antibody, RecQ protein-like 3 antibody, RecQ-like type 2 antibody, RecQ2 antibody, RECQL 2 antibody, RECQL 3 antibody, RECQL2 antibody, RECQL3 antibody, type 2 antibody

UniProt: P54132

Pathways: DNA Damage Repair