FLT3 antibody (pTyr842)

Catalog No. ABIN7139079

The Rabbit Polyclonal anti-FLT3 antibody has been validated for WB and ELISA. It is suitable to detect FLT3 in samples from Human.

Quick Overview for FLT3 antibody (pTyr842) (ABIN7139079)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FLT3 Antibody

Binding Specificity: pTyr842

Cross-Reactivity: Human, Mouse

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 842(S-N-Y(p)-V-V) derived from Human FLT3 .

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for FLT3

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: FLT3

Background:

Background:

FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Small D., Proc. Natl. Acad. Sci. U.S.A. 91:459-463(1994).
Rosnet O., Blood 82:1110-1119(1993).
Rosnet O., Genomics 9:380-385(1991).

Aliases: CD 135 antibody, CD135 antibody, CD135 antigen antibody, Fetal liver kinase 2 antibody, FL cytokine receptor antibody, Flk 2 antibody, Flk2 antibody, Flt 3 antibody, FLT-3 antibody, Flt3 antibody, FLT3_HUMAN antibody, FMS like tyrosine kinase 3 antibody, Fms related tyrosine kinase 3 antibody, Fms-like tyrosine kinase 3 antibody, Growth factor receptor tyrosine kinase type III antibody, Ly-72 antibody, OTTHUMP0000004234 antibody, Receptor type tyrosine protein kinase FLT3 antibody, Stem cell tyrosine kinase 1 antibody, Stk 1 antibody, STK-1 antibody, Stk1 antibody, Tyrosine protein kinase receptor FLT3 antibody, Tyrosine-protein kinase receptor FLT3 antibody

UniProt: P36888

Pathways: RTK Signaling