FEN1 antibody (AA 85-383) (Biotin)

Catalog No. ABIN7140479

This Rabbit Polyclonal antibody specifically detects FEN1 in ELISA. It exhibits reactivity toward Arabidopsis thaliana.

Quick Overview for FEN1 antibody (AA 85-383) (Biotin) (ABIN7140479)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEN1 antibody is conjugated to Biotin

Application: ELISA

Product Details anti-FEN1 Antibody (Biotin)

Binding Specificity: AA 85-383

Cross-Reactivity: Arabidopsis thaliana

Purification: >95%, Protein G purified

Immunogen: Recombinant Arabidopsis thaliana Flap endonuclease 1 protein (85-383AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: FEN1

Background:

Background: Structure-specific nuclease with 5\'-flap endonuclease and 5\'-3\' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5\'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5\'-end of a downstream Okazaki fragment. It enters the flap from the 5\'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinicapyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5\'-3\' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Aliases: FEN1 antibody, At5g26680 antibody, F21E10.3Flap endonuclease 1 antibody, FEN-1 antibody, EC 3.1.-.- antibody, Flap structure-specific endonuclease 1 antibody

UniProt: O65251

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA