MSH2 antibody (AA 2-254) (HRP)

Catalog No. ABIN7150208

This Rabbit Polyclonal antibody specifically detects MSH2 in ELISA. It exhibits reactivity toward Human.

Quick Overview for MSH2 antibody (AA 2-254) (HRP) (ABIN7150208)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSH2 antibody is conjugated to HRP

Application: ELISA

Product Details anti-MSH2 Antibody (HRP)

Binding Specificity: AA 2-254

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human DNA mismatch repair protein Msh2 protein (2-254AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: MSH2

Background:

Background: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Aliases: BAT26 antibody, COCA 1 antibody, COCA1 antibody, DNA mismatch repair protein Msh2 antibody, FCC 1 antibody, FCC1 antibody, hMSH2 antibody, HNPCC 1 antibody, HNPCC antibody, HNPCC1 antibody, LCFS2 antibody, MSH 2 antibody, Msh2 antibody, MSH2_HUMAN antibody, MutS homolog 2 antibody, MutS homolog 2 colon cancer nonpolyposis type 1 antibody, MutS protein homolog 2 antibody

UniProt: P43246

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response