NEDD4-2 antibody (AA 289-494)

Catalog No. ABIN7151078

This Rabbit Polyclonal antibody specifically detects NEDD4-2 in ELISA, WB and IF. It exhibits reactivity toward Human.

Quick Overview for NEDD4-2 antibody (AA 289-494) (ABIN7151078)

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD4-2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-NEDD4-2 Antibody

Binding Specificity: AA 289-494

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human E3 ubiquitin-protein ligase NEDD4-like protein (289-494AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for NEDD4-2

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Alternative Name: NEDD4L

Background:

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, KCNH2, EAAT1 or CLC5 (PubMed:26363003). Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046). Plays a role in dendrite formation by melanocytes (PubMed:23999003). Involved in the regulation of TOR signaling (PubMed:27694961).

Aliases: E3 ubiquitin protein ligase NEDD4 like protein antibody, E3 ubiquitin-protein ligase NEDD4-like antibody, KIAA0439 antibody, NED4L_HUMAN antibody, NEDD4 2 antibody, NEDD4 2c antibody, Nedd4-2 antibody, NEDD4-2a antibody, NEDD4.2 antibody, NEDD4b antibody, NEDD4L antibody, NEDD4La antibody, NEDD4Lb antibody, NEDD4Lc antibody, NEDD4Ld antibody, NEDD4Le antibody, NEDD4Lf antibody, NEDD4Lg antibody, NEDD4Lh antibody, NEDL3 antibody, Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody, Neural precursor cell expressed, developmentally down regulated 4 like antibody, RSP5 antibody, Ubiquitin protein ligase Rsp5 antibody

UniProt: Q96PU5

Pathways: Negative Regulation of Transporter Activity