TIGAR antibody (AA 220-270)

Catalog No. ABIN7454443

The Rabbit Polyclonal anti-TIGAR antibody is suitable to detect TIGAR in samples from Human. It has been validated for IP and IHC (fp).

Quick Overview for TIGAR antibody (AA 220-270) (ABIN7454443)

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIGAR antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-TIGAR Antibody

Binding Specificity: AA 220-270

Purpose: Rabbit anti-TIGAR Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 220 and 270

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 10 μg/mg lysate

WB: Not recommended. Use rabbit anti-TIGAR antibody A302-285A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for TIGAR

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Alternative Name: TIGAR

Background: Background: TIGAR (TP53-induced glycolysis and apoptosis regulator) is a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis [taken from NCBI GeneID:57103].

Gene ID: 57103

NCBI Accession: NP_065108

UniProt: Q9NQ88

Pathways: Warburg Effect