Paxillin antibody (pSer178)

Catalog No. ABIN711221

The Rabbit Polyclonal anti-Paxillin antibody has been validated for WB, ELISA, IF (cc), IF (p) and IHC (fro). It is suitable to detect Paxillin in samples from Human. There is 1 publication available.

Quick Overview for Paxillin antibody (pSer178) (ABIN711221)

Target: Paxillin (PXN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Paxillin Antibody

Binding Specificity: pSer178

Predicted Reactivity: Human,Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Ser178

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Paxillin antibody (ABIN711221)

Stelling-Férez, Gabaldón, Nicolás: "Oleanolic acid stimulation of cell migration involves a biphasic signaling mechanism." in: Scientific reports, Vol. 12, Issue 1, pp. 15065, (2022) (PubMed).

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: Paxillin

Background:

Synonyms: PXNphospho S178, Paxillin phospho S178, Paxillin phospho Ser178, p-Paxillin Ser178, Paired box protein Pax 1, PAX 1, PAX1, Paxillin alpha, PXN, PXN protein, FLJ16691, FLJ23042, FLJ16691, PAXI_HUMAN, Paxillin, PXN protein.

Background: Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).

Gene ID: 5829

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling