CHEK2 antibody (AA 1-270)

Catalog No. ABIN7169027

This Rabbit Polyclonal antibody specifically detects CHEK2 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CHEK2 antibody (AA 1-270) (ABIN7169027)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-CHEK2 Antibody

Binding Specificity: AA 1-270

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Serine/threonine-protein kinase Chk2 protein (1-270AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:200-1:500,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHEK2

Background:

Background: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at \\\'Ser-20\\\' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978).

Aliases: CDS 1 antibody, Cds1 antibody, Cds1 homolog antibody, Checkpoint kinase 2 antibody, Checkpoint like protein CHK2 antibody, CHEK 2 antibody, Chek2 antibody, Chk 2 antibody, CHK2 checkpoint homolog (S. pombe) antibody, CHK2 checkpoint homolog antibody, CHK2_HUMAN antibody, hCds1 antibody, HuCds 1 antibody, LFS 2 antibody, LFS2 antibody, PP1425 antibody, RAD 53 antibody, RAD53 antibody, Rad53 homolog antibody, Serine/threonine protein kinase Chk2 antibody, Serine/threonine-protein kinase Chk2 antibody

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle