NF-kB p65 antibody (pThr435) (Cy5)

Catalog No. ABIN712875

This anti-NF-kB p65 antibody is a Rabbit Polyclonal antibody detecting NF-kB p65 in WB, FACS, IF (cc) and IF (p). Suitable for Human and Mouse.

Quick Overview for NF-kB p65 antibody (pThr435) (Cy5) (ABIN712875)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is conjugated to Cy5

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-NF-kB p65 Antibody (Cy5)

Binding Specificity: pThr435

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human NFKBp65 around the phosphorylation site of Thr435

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: NFKB p65

Background:

Synonyms: NFkB-p65Thr435, RELAphospho T435, NFKBp65, NF-Bp65, p65 NF kappaB, p65 NFkB, NFKBp65, RELA, Transcription Factor p65, v rel avian reticuloendotheliosis viral oncogene homolog A nuclear factor of kappa light polypeptide gene enhancer in B cells 3 p65, V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A, v rel reticuloendotheliosis viral oncogene homolog A avian, v-rel reticuloendotheliosis viral oncogene homolog A, p65NFKB, Avian reticuloendotheliosis viral v rel oncogene homolog A, MGC131774, NFKB 3, NFKB3, Nuclear Factor NF Kappa B p65 Subunit, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells.

Background: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].

Gene ID: 5970

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins