RAG2 antibody (AA 296-415) (Biotin)

Catalog No. ABIN7175271

Product Details anti-RAG2 Antibody (Biotin)

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Binding Specificity: AA 296-415

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAG2 antibody is conjugated to Biotin

Application: ELISA

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human V(D)J recombination-activating protein 2 protein (296-415AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for RAG2

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Alternative Name: RAG2 (RAG2 Products)

Synonyms: RAG-2 antibody, Rag-2 antibody, rag-2 antibody, zgc:136743 antibody, recombination activating 2 antibody, recombination activating gene 2 antibody, recombination activating gene 2 S homeolog antibody, RAG2 antibody, Rag2 antibody, rag2 antibody, rag2.S antibody

Background:

Background: Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3\'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5\'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at \'Lys-4\' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity).

Aliases: RAG 2 antibody, RAG-2 antibody, RAG2 antibody, RAG2_HUMAN antibody, Recombination activating gene 2 antibody, V(D)J recombination activating protein 2 antibody, V(D)J recombination-activating protein 2 antibody

UniProt: P55895

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response