VPS4A antibody (AA 1-120)

Catalog No. ABIN7175319

The Rabbit Polyclonal anti-VPS4A antibody has been validated for WB, IHC and ELISA. It is suitable to detect VPS4A in samples from Human.

Quick Overview for VPS4A antibody (AA 1-120) (ABIN7175319)

Target: VPS4A (Vacuolar Protein Sorting-Associated Protein 4A (VPS4A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-VPS4A Antibody

Binding Specificity: AA 1-120

Cross-Reactivity: Human, Mouse, Rat

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Vacuolar protein sorting-associated protein 4A protein (1-120AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for VPS4A

Target: VPS4A (Vacuolar Protein Sorting-Associated Protein 4A (VPS4A))

Alternative Name: VPS4A

Background:

Background: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Aliases: hVPS4 antibody, Protein SKD2 antibody, SKD1 antibody, SKD1 homolog antibody, SKD1A antibody, SKD2 antibody, Vacuolar protein sorting 4 homolog A antibody, Vacuolar protein sorting 4, yeast, homolog of, A antibody, Vacuolar protein sorting factor 4A antibody, Vacuolar protein sorting-associated protein 4A antibody, vacuolar sorting protein 4 antibody, VPS4 antibody, VPS4-1 antibody, Vps4a antibody, VPS4A_HUMAN antibody

UniProt: Q9UN37

Pathways: Microtubule Dynamics, CXCR4-mediated Signaling Events