MAP2K4 antibody (AA 2-397)

Catalog No. ABIN7177157

This Rabbit Polyclonal antibody specifically detects MAP2K4 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for MAP2K4 antibody (AA 2-397) (ABIN7177157)

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MAP2K4 Antibody

Binding Specificity: AA 2-397

Cross-Reactivity: Human, Mouse, Rat

Purification: >95%, Protein G purified

Immunogen: Recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 4 protein (2-397AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:5000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MAP2K4

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Alternative Name: Map2k4

Background:

Background: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.

Aliases: Map2k4 antibody, Jnkk1 antibody, Mek4 antibody, Mkk4 antibody, Prkmk4 antibody, Sek1 antibody, Serk1 antibody, Skk1 antibody, Dual specificity mitogen-activated protein kinase kinase 4 antibody, MAP kinase kinase 4 antibody, MAPKK 4 antibody, EC 2.7.12.2 antibody, C-JUN N-terminal kinase kinase 1 antibody, JNK kinase 1 antibody, JNKK 1 antibody, JNK-activating kinase 1 antibody, MAPK/ERK kinase 4 antibody, MEK 4 antibody, SAPK/ERK kinase 1 antibody, SEK1 antibody

UniProt: P47809

Pathways: MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling