APLF antibody (Ser116)

Catalog No. ABIN7179974

This Rabbit Polyclonal antibody specifically detects APLF in ELISA, WB and IHC. It exhibits reactivity toward Human.

Quick Overview for APLF antibody (Ser116) (ABIN7179974)

Target: APLF (Aprataxin and PNKP Like Factor (APLF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APLF antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-APLF Antibody

Binding Specificity: Ser116

Purpose: APLF (Ab-116) Antibody

Cross-Reactivity: Mouse

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from Human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V).

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for APLF

Target: APLF (Aprataxin and PNKP Like Factor (APLF))

Alternative Name: APLF

Background:

Background: Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.

Saunders W.S., J. Cell Sci. 104:573-582(1993).
Ye Q., J. Biol. Chem. 271:14653-14656(1996).
Ye Q., J. Biol. Chem. 272:14983-14989(1997).

Aliases: 2010301N04Rik antibody, AI452191 antibody, Aplf antibody, APLF_HUMAN antibody, Aprataxin and PNK-like factor antibody, Apurinic-apyrimidinic endonuclease APLF antibody, C2orf13 antibody, PNK and APTX like FHA protein antibody, PNK and APTX-like FHA domain-containing protein antibody, RGD1565557 antibody, XIP1 antibody, XRCC1 interacting protein 1 antibody, XRCC1-interacting protein 1 antibody

UniProt: Q8IW19

Pathways: DNA Damage Repair