p300 antibody

Catalog No. ABIN7182230

This Rabbit Polyclonal antibody specifically detects p300 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for p300 antibody (ABIN7182230)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p300 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-p300 Antibody

Purpose: EP300/CREBBP Antibody

Cross-Reactivity: Mouse, Rat

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from Human P300/CBP.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: EP300

Background:

Background: Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers.

Gerd A. Blobel, J. Leukoc. Biol., Apr 2002, 71: 545.
Eric Kalkhoven, Hum. Mol. Genet., Feb 2003, 12: 441 - 450.
Wei-fang Shen, Mol. Cell. Biol., Nov 2001, 21: 7509 - 7522.
Frederick C. Nucifora, Science, Mar 2001, 291: 2423.

Aliases: E1A associated protein p300 antibody, E1A binding protein p300 antibody, E1A-associated protein p300 antibody, EP300 antibody, EP300: E1A binding protein p300 antibody, EP300_HUMAN antibody, Histone acetyltransferase p300 antibody, KAT3B antibody, p300 HAT antibody, RSTS2 antibody

UniProt: Q09472

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size