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Vimentin antibody (AA 2-466)

Name: Vimentin antibody (AA 2-466)
Brand: antibodies-online
SKU: ABIN7193582
Price: 533.5 USD
Availability: InStock

The Mouse Monoclonal anti-Vimentin antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect Vimentin in samples from Human, Mouse and Rat.

Western blot analysis using VIM mAb against HEK293 (1) and VIM (AA: 2-466)-hIgGFc transfected HEK293 (2) cell lysate. (Vimentin antibody (AA 2-466))

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using VIM mouse mAb with DAB staining. (Vimentin antibody (AA 2-466))

IHC

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (Vimentin antibody (AA 2-466))

ELISA

Western blot analysis using VIM mAb against human VIM (AA: 2-466) recombinant protein. (Vimentin antibody (AA 2-466))

Western blot analysis using VIM mouse mAb against Jurkat (1), K562 (2), SK-N-SH (3), SH-SY5Y (4), Hela (5), NIH/3T3 (6), C6 (7), and RAW264. (Vimentin antibody (AA 2-466))

Flow cytometric analysis of Hela cells using VIM mouse mAb (green) and negative control (red). (Vimentin antibody (AA 2-466))

FACS

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using VIM mouse mAb with DAB staining. (Vimentin antibody (AA 2-466))

IHC

7 images

Catalog No. ABIN7193582

Quick Overview for Vimentin antibody (AA 2-466) (ABIN7193582)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Reactivity

Human, Mouse, Rat

Host

Mouse

Clonality

Monoclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone

3A1F2

Discover our top product VIM Primary Antibody

Binding Specificity
32

30

17

16

12

10

9

7

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-466

Purpose

Vimentin Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human VIM (AA: 2-466) expressed in E. Coli.

Isotype

IgG2a
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated

Application Notes

ELISA: 1/10000
FCM: 1/200-1/400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Target

Vimentin (VIM)

Alternative Name

Vimentin

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Molecular Weight

53.7 kDa

Gene ID

7431

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis