ERCC1 antibody (AA 1-297)

Catalog No. ABIN7193699

The Mouse Monoclonal anti-ERCC1 antibody is suitable to detect ERCC1 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and FACS.

Quick Overview for ERCC1 antibody (AA 1-297) (ABIN7193699)

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 5A2D1

Product Details anti-ERCC1 Antibody

Binding Specificity: AA 1-297

Purpose: ERCC1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human ERCC1 (AA: 1-297) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200-1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1

Background:

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Molecular Weight: 32.5 kDa

UniProt: P07992

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response