PFKFB2 antibody (pSer483)

Catalog No. ABIN7213270

The Rabbit Polyclonal anti-PFKFB2 antibody (ABIN7213270) specifically detects PFKFB2 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PFKFB2 antibody (pSer483) (ABIN7213270)

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKFB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PFKFB2 Antibody

Binding Specificity: pSer483

Purpose: Rabbit Anti-PFK-2 car (phospho Ser483) Polyclonal Antibody

Specificity: Phospho-PFK-2 car (S483) Polyclonal Antibody detects endogenous levels of PFK-2 car protein only when phosphorylated at S483.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PFK-2 car Phospho-Ser483

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PFKFB2

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Alternative Name: PFK-2 car

Background: PFKFB2, 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2, 6PF-2-K/Fru-2,6-P2ase 2, PFK/FBPase 2, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 encoded by PFKFB2 is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for PFKFB2.

Molecular Weight: 58kD

Gene ID: 5208

UniProt: O60825

Pathways: PI3K-Akt Signaling, Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process