AKAP12 antibody (AA 270-350)

Catalog No. ABIN7226038

The Rabbit Polyclonal anti-AKAP12 antibody (ABIN7226038) specifically detects AKAP12 in IHC, ELISA and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for AKAP12 antibody (AA 270-350) (ABIN7226038)

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP12 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-AKAP12 Antibody

Binding Specificity: AA 270-350

Purpose: Rabbit Anti-AKAP 250 Polyclonal Antibody

Specificity: AKAP 250 Polyclonal Antibody detects endogenous levels of AKAP 250 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human AKAP 250 at AA range: 270-350

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AKAP12

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Alternative Name: AKAP 250

Background: AKAP12, AKAP250, A-kinase anchor protein 12, AKAP-12, A-kinase anchor protein 250 kDa, AKAP 250, Gravin, Myasthenia gravis autoantigenThe A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. AKAP12 (A-kinase anchoring protein 12) encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Gene ID: 9590

UniProt: Q02952

Pathways: cAMP Metabolic Process