AKAP8 antibody (AA 300-380)

Catalog No. ABIN7213528

The Rabbit Polyclonal anti-AKAP8 antibody (ABIN7213528) specifically detects AKAP8 in WB, IHC, IF and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for AKAP8 antibody (AA 300-380) (ABIN7213528)

Target: AKAP8 (A Kinase (PRKA) Anchor Protein 8 (AKAP8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-AKAP8 Antibody

Binding Specificity: AA 300-380

Purpose: Rabbit Anti-AKAP 95 Polyclonal Antibody

Specificity: AKAP 95 Polyclonal Antibody detects endogenous levels of AKAP 95 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human AKAP 95 at AA range: 300-380

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AKAP8

Target: AKAP8 (A Kinase (PRKA) Anchor Protein 8 (AKAP8))

Alternative Name: AKAP 95

Background: AKAP8, AKAP95, A-kinase anchor protein 8, AKAP-8, A-kinase anchor protein 95 kDa, AKAP 95AKAP8 encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. ThAKAP8 encodes a nuclear A-kinase anchor protein (A-kinase anchoring protein 8) that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of AKAP8 is located on the short arm of chromosome 9.

Molecular Weight: 76kD

Gene ID: 10270

UniProt: O43823

Pathways: SARS-CoV-2 Protein Interactome