ACTN3 antibody (AA 10-90)

Catalog No. ABIN7213472

This Rabbit Polyclonal antibody specifically detects ACTN3 in WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ACTN3 antibody (AA 10-90) (ABIN7213472)

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-ACTN3 Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-Actinin-α3 Polyclonal Antibody

Specificity: Actinin-α3 Polyclonal Antibody detects endogenous levels of Actinin-α3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Actinin-alpha3 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ACTN3

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Alternative Name: Actinin-alpha3

Background: ACTN3, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking proteinACTN3 encodes a member of the alpha-actin binding protein gene family. Actinin alpha 3 (gene/pseudogene) is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants, the reference genome represents the coding allele. The non-functional allele of ACTN3 is associated with elite athlete status.

Molecular Weight: 103kD

Gene ID: 89

UniProt: Q08043

Pathways: Cell-Cell Junction Organization