CD13 antibody (C-Term)

Catalog No. ABIN7218662

The Rabbit Polyclonal anti-CD13 antibody is suitable to detect CD13 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for CD13 antibody (C-Term) (ABIN7218662)

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CD13 Antibody

Binding Specificity: C-Term

Purpose: Rabbit Anti-CD13 Polyclonal Antibody

Specificity: CD13 Polyclonal Antibody detects endogenous levels of CD13 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human CD13

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD13

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: CD13

Background: ANPEP, APN, CD13, PEPN, Aminopeptidase N, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.

Molecular Weight: 110kD

Gene ID: 290

UniProt: P15144

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones