ATP1A1 antibody (pTyr260)

Catalog No. ABIN7231444

The Rabbit Polyclonal anti-ATP1A1 antibody is suitable to detect ATP1A1 in samples from Human, Rat and Mouse. It has been validated for WB, IHC and ELISA.

Quick Overview for ATP1A1 antibody (pTyr260) (ABIN7231444)

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-ATP1A1 Antibody

Binding Specificity: pTyr260

Purpose: Rabbit Anti-Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Polyclonal Antibody

Specificity: The antibody detects endogenous levels of Sodium Potassium ATPase alpha-1 when Phospho occurs at Tyr260

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Antibody protein at AA range: 230-290

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Alternative Name: Sodium Potassium ATPase alpha-1

Background: Sodium/potassium-transporting ATPase subunit alpha-1, Na(+/K(+ ATPase alpha-1 subunit, Sodium pump subunit alpha-1The protein encoded by ATP1A1 belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. ATP1A1 encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for ATP1A1.

Molecular Weight: 115kD

Gene ID: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process