alpha Tubulin antibody

Catalog No. ABIN7212010

The Mouse Monoclonal anti-alpha Tubulin antibody has been validated for WB. It is suitable to detect alpha Tubulin in samples from Zebrafish (Danio rerio).

Quick Overview for alpha Tubulin antibody (ABIN7212010)

Target: alpha Tubulin (TUBA1)

Reactivity: Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-alpha Tubulin Antibody

Purpose: α-tubulin Monoclonal Antibody

Specificity: The antibody detects Zebrafish endogenousα-tubulin protein.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Immunogen: Recombinant Protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:10000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha-tubulin

Background: Mouse Anti-α-tubulin Monoclonal Antibody,TUBB3, TUBB4, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III,Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. TUBA1A encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that TUBA1A expression is predominantly found in morphologically differentiated neurologic cells. TUBA1A is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in TUBA1A cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.,Tubulin alpha-1A chain

Molecular Weight: observerd band 52kDa

Gene ID: 10381

UniProt: Q13509

Pathways: Microtubule Dynamics