alpha Tubulin antibody (acLys40)

Catalog No. ABIN7212140

This Mouse Monoclonal antibody specifically detects alpha Tubulin in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for alpha Tubulin antibody (acLys40) (ABIN7212140)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5H5

Product Details anti-alpha Tubulin Antibody

Binding Specificity: acLys40

Purpose: α-tubulin (Acetyl Lys40) Monoclonal Antibody (5H5)

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Immunogen: Synthetic Peptide

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:100-1:200).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha-tubulin

Background: Mouse Anti-α-tubulin (Acetyl Lys40) Monoclonal Antibody (5H5),Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. TUBA4A (tubulin alpha 4a) encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for TUBA4A.,α-tubulin (Acetyl Lys40)

Molecular Weight: observerd band 52kDa

Gene ID: 7277

UniProt: P68363

Pathways: Microtubule Dynamics