Ki-67 antibody (AA 3170-3250)

Catalog No. ABIN7215534

The Rabbit Polyclonal anti-Ki-67 antibody (ABIN7215534) specifically detects Ki-67 in IHC, IF and ELISA. The antibody is reactive with Human samples.

Quick Overview for Ki-67 antibody (AA 3170-3250) (ABIN7215534)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-Ki-67 Antibody

Binding Specificity: AA 3170-3250

Purpose: Rabbit Anti-Ki-67 Polyclonal Antibody

Specificity: Ki-67 Polyclonal Antibody detects endogenous levels of Ki-67 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Ki-67 at AA range: 3170-3250

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki-67

Background: MKI67, Antigen KI-67MKI67 encodes Antigen KI-67 that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

Molecular Weight: 360kD

Gene ID: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process