Apoptosis Inhibitor 5 antibody (AA 390-470)

Catalog No. ABIN7213626

The Rabbit Polyclonal anti-Apoptosis Inhibitor 5 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect Apoptosis Inhibitor 5 in samples from Human and Mouse.

Quick Overview for Apoptosis Inhibitor 5 antibody (AA 390-470) (ABIN7213626)

Target: Apoptosis Inhibitor 5 (API5)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Apoptosis Inhibitor 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Apoptosis Inhibitor 5 Antibody

Binding Specificity: AA 390-470

Purpose: API5 Polyclonal Antibody

Specificity: API5 Polyclonal Antibody detects endogenous levels of API5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human API5 at AA range: 390-470

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Apoptosis Inhibitor 5

Target: Apoptosis Inhibitor 5 (API5)

Alternative Name: API5

Background: Rabbit Anti-API5 Polyclonal Antibody,API5, MIG8, Apoptosis inhibitor 5, API-5, Antiapoptosis clone 11 protein, AAC-11, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, Protein XAGL,API5 encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein (apoptosis inhibitor 5) suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.,Apoptosis inhibitor 5

Gene ID: 8539

UniProt: Q9BZZ5

Pathways: Growth Factor Binding