ADRA1B antibody (AA 410-490)

Catalog No. ABIN7219784

The Rabbit Polyclonal anti-ADRA1B antibody (ABIN7219784) specifically detects ADRA1B in WB, ELISA and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for ADRA1B antibody (AA 410-490) (ABIN7219784)

Target: ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRA1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-ADRA1B Antibody

Binding Specificity: AA 410-490

Purpose: Rabbit Anti-AR-α1B Polyclonal Antibody

Specificity: AR-α1B Polyclonal Antibody detects endogenous levels of AR-α1B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human AR-alpha1B at AA range: 410-490

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ADRA1B

Target: ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))

Alternative Name: AR-alpha1B

Background: ADRA1B, Alpha-1B adrenergic receptor, Alpha-1B adrenoreceptor, Alpha-1B adrenoceptorAlpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. ADRA1B encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. ADRA1B comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region.

Molecular Weight: 60kD

Gene ID: 147

UniProt: P35368

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process